Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals

Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals

Ideally, an unpaired electron shows a single peak in the EPR spectrum due to the transition between the two spin energy states. However, coupling interactions can occur between the spins of the unpaired electron and any neighboring spin-active nuclei. This hyperfine coupling results in hyperfine splitting, where the EPR signal is split into multiplets. The signals split into 2nI + 1 peaks, where n is the number of equivalent nuclei and I is the nuclear spin. These splitting patterns provide...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Topology-Dependent Coke Formation in the Catalytic Pyrolysis of Phenol Over HFAU and HZSM-5 Zeolites.

Angewandte Chemie (International ed. in English)·2026

Same author

Characterization of strongly hyperfine-split protons by DNP.

Physical chemistry chemical physics : PCCP·2026

Same author

Characterization of flexible RNA binding by tandem RNA recognition motifs through integrative ensemble modelling.

Nucleic acids research·2026

Same author

Single atoms of indium on hafnia enable superior CO<sub>2</sub>-based methanol synthesis.

Nature nanotechnology·2026

Same author

Towards full optimisation of automated double electron-electron resonance spectroscopy.

Physical chemistry chemical physics : PCCP·2025

Same author

Tracking Chromium Evolution on Ceria from Particles to Single Atoms: A Catalyst Regeneration Strategy for Ammonia Oxidation.

Journal of the American Chemical Society·2025

Same journal

Localization-driven exchange contrast in diffusion exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026

Same journal

4.5 Tesla superconducting miniature magnet in liquid nitrogen.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026

Same journal

Folding and unfolding dynamics of a DNA aptamer studied by heteronuclear <sup>1</sup>H-<sup>13</sup>C correlation zz-exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026

Same journal

Multi-spin control from one-spin pulses.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026

Same journal

Altering MRI rotating frame relaxations by changing the truncation level of Hyperbolic Secant pulse.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026

Same journal

Effects of proton exchange on the lifetimes of long-lived states in aliphatic chains.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 21, 2026

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1

Published on: November 26, 2014

Quo vadis EPR?

Gunnar Jeschke¹

¹ETH Zurich, Lab. Phys. Chem., Vladimir-Prelog-Weg 2, 8093 Zurich, Switzerland.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)

|July 27, 2019

Summary

This summary is machine-generated.

Electron Paramagnetic Resonance (EPR) spectroscopy needs enhanced data information content due to increasing system complexity. A third wave of method development, driven by digital electronics and computing, promises significant advancements in EPR analysis.

Keywords:

Catalysis Dynamical decoupling Integrative structural biology Machine learning Miniaturization Rapid scan Shaped pulses Spin dynamics

More Related Videos

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

Published on: March 16, 2018

Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds

Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds

Published on: October 18, 2018

Related Experiment Videos

Last Updated: Jan 21, 2026

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1

Published on: November 26, 2014

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

Published on: March 16, 2018

Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds

Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds

Published on: October 18, 2018

Area of Science:

Analytical Chemistry
Biophysics
Materials Science

Background:

Increasing complexity in paramagnetic catalysts, materials, and integrative structural biology systems necessitates enhanced data analysis.
Current Electron Paramagnetic Resonance (EPR) spectroscopy methods face limitations in extracting sufficient information from complex systems.

Purpose of the Study:

To propose a new wave of method development in EPR spectroscopy to enhance data information content.
To explore how advances in digital electronics and computing can drive innovation in EPR methodology.

Main Methods:

Leveraging recent advances in digital electronics and computing for EPR spectroscopy.
Adapting Nuclear Magnetic Resonance (NMR) methods for application in EPR.
Developing novel conceptual approaches for EPR data acquisition and analysis.

Main Results:

Anticipates a significant enhancement in the information content obtainable from EPR data.
Suggests a dual approach combining established NMR transfer techniques with entirely new EPR concepts.
Highlights the potential for transformative improvements in understanding complex paramagnetic systems.

Conclusions:

A third major wave of method development in EPR spectroscopy is emerging, driven by technological advancements.
This new wave will enable deeper insights into complex paramagnetic systems, crucial for catalysis and structural biology.
Future EPR methodology will integrate established techniques with innovative, system-specific approaches.