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Updated: Jan 21, 2026
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A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene
Published on: April 1, 2019
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Development of polymorphic simple sequence repeat markers in Huperzia serrata (Lycopodiaceae)
Bin Guo1, Jing-Yu Ren1, Mei-Na He1
1Key Laboratory of Resource Biology and Biotechnology in Western China Department of Life Science Northwest University Xi'an 710069 People's Republic of China.
Applications in Plant Sciences
|July 27, 2019
Summary
Simple sequence repeat (SSR) markers were developed for the endangered Huperzia serrata. These markers reveal genetic diversity and population structure, aiding conservation efforts for this overexploited plant species.
Area of Science:
- Botany
- Conservation Genetics
- Molecular Ecology
Background:
- Huperzia serrata, a member of Lycopodiaceae, faces endangerment due to overexploitation.
- Assessing genetic diversity and population structure is crucial for conservation strategies.
Purpose of the Study:
- To develop simple sequence repeat (SSR) markers for Huperzia serrata.
- To utilize these markers for surveying genetic diversity and population structure.
Main Methods:
- Developed 120 SSR primer pairs from transcriptomic data of H. serrata.
- Optimized and screened primer pairs for polymorphism.
- Analyzed genetic diversity using 10 polymorphic loci across 177 individuals.
Main Results:
- 10 polymorphic SSR loci were identified, showing 2-11 alleles per locus.
- Observed heterozygosity ranged from 0.00 to 1.00; expected heterozygosity ranged from 0.19 to 0.79.
- Developed markers were also amplified in related species (H. crispata, H. sutchueniana, H. selago).
Conclusions:
- The 10 developed polymorphic SSR primer pairs are effective for studying H. serrata.
- These markers will facilitate future research on the genetic diversity and population structure of this endangered species and related taxa.
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