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Updated: Jan 21, 2026
Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial
Published on: September 29, 2015
Impact of different platelet-rich fibrin (PRF) procurement methods on the platelet count, antimicrobial efficacy, and
Alefiya S Mamajiwala1, Kunal S Sethi1, Chetan P Raut2
1Department of Periodontology, Karmaveer Bhausaheb Hiray Dental College and Hospital, Nashik, India.
Insights
Platelet-rich fibrin (PRF) properties vary with preparation methods and patient age. Lower centrifugation speeds and shorter times enhance platelet count and antimicrobial activity but reduce fibrin density.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Oral Surgery
Background:
- Platelet-rich fibrin (PRF) is a bioactive concentrate used in regenerative medicine.
- PRF preparation protocols, including centrifugation speed and duration, can influence its characteristics.
- Patient age may also impact the properties of PRF.
Purpose of the Study:
- To investigate how varying centrifugation speeds and durations affect PRF's fibrin network, platelet count, and antimicrobial efficacy.
- To evaluate the influence of different age groups on these PRF properties.
Main Methods:
- Sixty healthy subjects were divided into three age groups (20-34, 35-49, 50-65 years).
- PRF membranes were prepared using 10 ml tubes subjected to centrifugation at 1400, 2800, or 3500 rpm for either 8 or 15 minutes.
- PRF membranes were analyzed for platelet count, antimicrobial activity against oral bacteria, and fibrin network pattern.
Main Results:
- The 20-34 year age group yielded PRF with the highest platelet concentration, antimicrobial activity, and densest fibrin network.
- Lower centrifugation speeds (1400 rpm) and shorter times (8 min) resulted in increased platelet count and antimicrobial activity.
- Higher speeds and longer durations (3500 rpm for 15 min) produced a denser fibrin network.
Conclusions:
- PRF characteristics are significantly influenced by centrifugation parameters (speed, time, RCF) and patient age.
- Optimizing PRF preparation protocols is crucial for maximizing desired properties like platelet yield and antimicrobial effect.
- Tailoring centrifugation protocols based on patient age and desired therapeutic outcome is recommended.
Objectives:
Platelet-rich fibrin (PRF) procuring protocols have been suggested, differing in speed and time duration. Since different derivation protocols may alter PRF characteristics, the present study was conducted to evaluate the variations in the fibrin network pattern, platelet count, and antimicrobial efficacy of PRF procured using variable centrifugation speeds and time durations in different age groups.
Materials And Methods:
Sixty healthy subjects participated in the study and were equally divided into three age groups (20-34 years, 35-49 years, 50-65 years). From each age group, total of 6 PRF membranes were fabricated from 10 ml tubes. Three PRF membranes were obtained at 1400, 2800, and 3500 rpm for 8 min while other 3 membranes were obtained after 15 min of centrifugation respectively. The relative centrifugal force (RCF) values were within the spectrum of 228-1425 g. PRF membranes were then subjected to platelet count estimation, antimicrobial activity against oral bacteria, and changes in fibrin network pattern with respect to different age groups and different centrifugation protocols.
Results:
Highest platelet concentration, antimicrobial activity, and dense fibrin network were obtained in 20-34 years age group. Intragroup analysis within each group revealed highest platelet count and antimicrobial activity in PRF membranes, obtained at 1400 rpm for 8 min. Denser fibrin network pattern was demonstrated by PRF membranes procured at 3500 rpm for 15 min.
Conclusions:
PRF properties, i.e., platelet count, antimicrobial efficacy, and fibrin network, are influenced by technical aspects of PRF preparation (RCF value, centrifugation speed, and time) and patient age.
Clinical Relevance:
Based on the finding of present study, it can be implied that lower centrifugation speed and time can increase the platelet concentration and antimicrobial activity of the PRF membrane. Contrarily, lowering the speed and time leads to lesser density fibrin network pattern. Centrifugation protocols thus need to be adapted accordingly.
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