[Effect of pyruvate on metabolic processes in erythrocytes after acute massive blood loss]

It is shown in the in vitro experiments that addition into the incubation medium of 10 mumol pyruvate is accompanied by the inhibition of the lactate dehydrogenase activity. The level of 2.3-biphosphoglycerate, ATP, lactate and hemoglobin increases by 92%, 265%, 93% and 56%, respectively. The acute massive haemorrhage against a background of infusion of 0.065% solution of pyruvate does not evoke a decrease in the hemoglobin level, which is observed after infusion of the physiological solution. The value of hematocrit proved to be higher to some extent. The 2,3-biphosphoglycerate level and the activity of glucoso-6-phosphate dehydrogenase are not changed in comparison with the results of intact rats. The glutathione reductase activity rises by 38%. The lactate level diminishes by 34%. Pyruvate is established to exert a therapeutic effect in acute massive haemorrhage. The survival rate of rats after infusion of pyruvate increases by 65%.