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Microcalorimetric investigations on Listeria
F J Allerberger1, A Schulz, M P Dierich
1Institut für Hygiene, Universität Innsbruck, Austria.
Zentralblatt Fur Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology
|March 1, 1988
Summary
Microcalorimetry revealed distinct heat production patterns for Listeria ivanovii and Listeria seeligeri. Most other Listeria species, including Listeria monocytogenes, exhibited similar thermograms, differentiating them from the former two.
Area of Science:
- Microbiology
- Biophysics
Background:
- Listeria species are important foodborne pathogens.
- Differentiating Listeria species is crucial for food safety and diagnostics.
Purpose of the Study:
- To investigate the potential of microcalorimetry to differentiate between various Listeria species based on their heat production during growth.
- To establish characteristic thermograms for different Listeria species.
Main Methods:
- Sixty-one strains from five Listeria species (L. monocytogenes, L. ivanovii, L. innocua, L. welshimeri, L. seeligeri) were analyzed.
- Microcalorimetry was used to measure heat production during growth in Columbia broth.
- Thermograms were analyzed for characteristic patterns.
Main Results:
- Listeria ivanovii and Listeria seeligeri displayed unique and characteristic thermograms.
- Listeria welshimeri, Listeria innocua, and most Listeria monocytogenes strains showed similar microcalorimetric curves.
- The thermograms of L. ivanovii and L. seeligeri were significantly different from the other tested Listeria species.
Conclusions:
- Microcalorimetry can differentiate between certain Listeria species based on their metabolic heat signatures.
- Distinct thermograms were observed for L. ivanovii and L. seeligeri, suggesting potential for their specific identification.
- This technique offers a promising approach for rapid microbial analysis and differentiation within the Listeria genus.