Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

11.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.8K
Quantifying Work02:30

Quantifying Work

24.1K
As a system undergoes a change, its internal energy can change, and energy can be transferred from the system to the surroundings, or from the surroundings to the system.
24.1K
Cut-off Frequency of BJT01:17

Cut-off Frequency of BJT

1.4K
Cut-off frequencies in Bipolar Junction Transistors (BJTs) mark the transition between the signal's pass band and stop band, influencing their performance in amplifying or attenuating frequencies. These frequencies are crucial for designing BJTs to meet specific operational requirements in electronic circuits.
Alpha Cut-Off Frequency: Pertinent to the common-base configuration, the alpha cut-off frequency defines the upper-frequency limit at which the current gain, alpha, remains stable. As...
1.4K
Brick Cutting Techniques01:08

Brick Cutting Techniques

598
Brick-cutting techniques involve various tools and methods to shape bricks for construction. A mason's hammer with a chisel-pointed end is used for basic shaping through sharp, precise strikes. For more complex shapes requiring higher precision, a power saw with a water-cooled diamond blade is used.
Cut bricks are categorized by size. Bricks cut to half their original length are called half-bats, while those cut to three-fourths their length are known as three-fourth bats.
Special types of...
598
High-Level and Low-Level Awareness01:19

High-Level and Low-Level Awareness

631
Controlled processes in human consciousness represent high-alert mental states where individuals deliberately focus their attention on achieving specific goals. Controlled processes can be seen in situations like mastering new technology, where a person might become so absorbed that they ignore surrounding distractions. Such processes involve selective attention, requiring one to concentrate on particular elements of experience while disregarding others. These are governed by executive...
631
Leveling Effect01:29

Leveling Effect

1.4K
In acid-base chemistry, the leveling effect refers to the limitation imposed by the solvent on the strength of acids and bases in solution. When a base stronger than the solvent's conjugate base is used, it deprotonates the solvent until the base is entirely consumed, making it ineffective against weaker acids. Conversely, an acid stronger than the solvent's conjugate acid protonates the solvent until the acid is depleted, rendering it ineffective against weaker bases. Essentially, the...
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure of the MIWI endoribonuclease bound to pachytene piRNAs from mouse testes.

Cell reports·2026
Same author

In Vitro Biological Activities of an Endophytic Fungus, <i>Trichoderma</i> sp. L2D2 Isolated from <i>Anaphalis contorta</i>.

Indian journal of microbiology·2024
Same author

Roles of N<sup>6</sup>-methyladenosine writers, readers and erasers in the mammalian germline.

Current opinion in genetics & development·2024
Same author

Essential roles of RNA cap-proximal ribose methylation in mammalian embryonic development and fertility.

Cell reports·2023
Same author

The catalytic-dead Pcif1 regulates gene expression and fertility in <i>Drosophila</i>.

RNA (New York, N.Y.)·2023
Same author

Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery and exonuclease domain-containing proteins EXD1 and EXD2 in mouse spermatocytes.

Andrology·2023

Related Experiment Video

Updated: Jan 21, 2026

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

1.3K

Counting the Cuts: MAZTER-Seq Quantifies m6A Levels Using a Methylation-Sensitive Ribonuclease.

Radha Raman Pandey1, Ramesh S Pillai1

  • 1Department of Molecular Biology, Science III, University of Geneva, 30 Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland.

Cell
|July 27, 2019
PubMed
Summary

MAZTER-seq uses a novel enzyme to map N6-methyladenosine (m6A) methylation sites at single nucleotide resolution. This method identified numerous new sites and supports an m6A code influenced by local sequence context.

More Related Videos

A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure
09:06

A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure

Published on: October 24, 2018

11.2K
A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro
10:07

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro

Published on: May 17, 2017

17.4K

Related Experiment Videos

Last Updated: Jan 21, 2026

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

1.3K
A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure
09:06

A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure

Published on: October 24, 2018

11.2K
A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro
10:07

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro

Published on: May 17, 2017

17.4K

Area of Science:

  • Molecular Biology
  • Epigenetics
  • RNA Biology

Background:

  • N6-methyladenosine (m6A) is a prevalent RNA modification.
  • Understanding m6A distribution and regulation is crucial for deciphering gene expression.
  • Existing methods for m6A site mapping have limitations in resolution or scope.

Purpose of the Study:

  • To introduce MAZTER-seq, a new technique for nucleotide-resolution m6A quantification.
  • To identify novel m6A methylation sites.
  • To investigate the sequence determinants of m6A methylation.

Main Methods:

  • MAZTER-seq utilizes the sequence-specific, methylation-sensitive ribonuclease MazF.
  • MazF cleaves RNA at specific sites, with cleavage efficiency dependent on m6A presence.
  • High-throughput sequencing of cleaved RNA fragments allows for precise m6A site mapping.

Main Results:

  • MAZTER-seq provides single-nucleotide resolution of m6A sites.
  • The study identified a significant number of previously unknown m6A sites.
  • Methylation levels were found to be strongly correlated with local sequence composition.

Conclusions:

  • MAZTER-seq is a powerful tool for comprehensive m6A profiling.
  • The findings support the existence of a sequence-driven "m6A code."
  • Local sequence context is a primary determinant of m6A site methylation.