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Colorimetric determination of arprinocid in feed
Journal - Association of Official Analytical Chemists
|September 1, 1978
Summary
A new method accurately determines arprinocid in animal feed using colorimetric analysis after zinc reduction. This validated assay offers precise quantification of arprinocid, crucial for feed safety and quality control.
Area of Science:
- Analytical Chemistry
- Veterinary Pharmacology
Background:
- Arprinocid is an anticoccidial drug used in animal feed.
- Accurate quantification of arprinocid in feed is essential for efficacy and regulatory compliance.
Purpose of the Study:
- To develop and validate a sensitive analytical method for determining arprinocid concentration in animal feed.
- To assess potential interferences from common feed additives.
Main Methods:
- Extraction of arprinocid from feed using chloroform and phosphate buffer.
- Isolation via alumina adsorption chromatography and solvent partitioning.
- Colorimetric determination of arprinocid based on a diazo chromophore formed after zinc reduction.
Main Results:
- The method accurately quantifies arprinocid in feed within the 0.0010–0.0080% range.
- Achieved a precision of less than 5% relative standard deviation.
- Zoalene and sulfamethazine identified as significant interfering substances.
Conclusions:
- The developed analytical method provides a reliable means for arprinocid determination in feed.
- The method is suitable for routine quality control and regulatory monitoring of arprinocid levels.