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Continuous determination of effect of temperature on enzymic activities
T D Sherman1, J R Mahan, E A Funkhouser
1Department of Biochemistry and Biophysics, Texas A&M University, College Station 77843-2128.
Biochimie
|February 1, 1988
Abstract:
An electronic temperature controller was used to continuously change the temperature of cuvettes during enzymic assays. A microprocessor-controlled spectrophotometer continuously collected absorbance, time, and temperature data. Activation energies were calculated from Arrhenius plots of these data.