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Author Spotlight: Studying Behavior of Acanthamoeba to Develop Targeted Strategies for Preventing Acanthamoeba Keratitis
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Author Spotlight: Studying Behavior of Acanthamoeba to Develop Targeted Strategies for Preventing Acanthamoeba Keratitis

Published on: September 20, 2024

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In Vitro Evaluation of Combined Commercialized Ophthalmic Solutions Against Acanthamoeba Strains

María Reyes-Batlle1, Glorian Mura-Escorche1, Ines Sifaoui1

  • 1Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna. Av. Astrofísico Francisco Sánchez S/N, 38203 Tenerife, Spain.

Pathogens (Basel, Switzerland)
|July 28, 2019
PubMed

Insights

Novel ophthalmic solution mixtures show promise for treating Acanthamoeba infections. Combining Systane® Ultra and Naviblef® Daily Care demonstrated potent amoebicidal and cysticidal effects with low cytotoxicity, offering a potential therapeutic strategy for Acanthamoeba keratitis.

Area of Science:

  • Ophthalmology
  • Microbiology
  • Parasitology

Background:

  • Acanthamoeba species are ubiquitous protozoa causing severe human diseases like Acanthamoeba keratitis (AK).
  • Developing effective and safe treatments for AK is crucial due to its sight-threatening nature.
  • Evaluating existing commercial products offers a faster route to potential AK therapies.

Purpose of the Study:

  • To assess the amoebicidal and cysticidal activity of Systane® Ultra and Naviblef® Daily Care ophthalmic solutions.
  • To investigate the efficacy of combinations of these two commercial products against Acanthamoeba.
  • To evaluate the cytotoxic effects of these solutions on murine macrophages.

Main Methods:

  • Testing the anti-Acanthamoeba activity of individual ophthalmic solutions.
  • Evaluating synergistic effects of combined Systane® Ultra and Naviblef® Daily Care.
  • Assessing cytotoxicity against J774.1 murine macrophage cell line.

Main Results:

  • Naviblef® Daily Care exhibited significant activity against Acanthamoeba spp. individually.
  • Combinations of Systane® Ultra and Naviblef® Daily Care enhanced amoebicidal and cysticidal effects against Acanthamoeba castellanii.
  • Effective amoebicidal concentrations were substantially lower than cytotoxic concentrations for macrophages.

Conclusions:

  • Mixtures of Systane® Ultra and Naviblef® Daily Care demonstrate potent anti-Acanthamoeba activity.
  • These combinations present a promising therapeutic approach for Acanthamoeba infections, particularly AK.
  • The tested ophthalmic solution mixtures show a favorable safety profile with low cytotoxicity.
Keywords:
Acanthamoebaamoebicidal activitychemotherapykeratitisophthalmic solution

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