Related Experiment Video
Updated: Jan 21, 2026
MRI and PET in Mouse Models of Myocardial Infarction
Published on: December 19, 2013
Quantification of porcine myocardial perfusion with modified dual bolus MRI - a prospective study with a PET
Minna Husso1, Mikko J Nissi2, Antti Kuivanen3
1Diagnostic Imaging Center, Kuopio University Hospital, PO Box 100, 70029, Kuopio, KYS, Finland. minna.husso@kuh.fi.
Background:
The reliable quantification of myocardial blood flow (MBF) with MRI, necessitates the correction of errors in arterial input function (AIF) caused by the T1 saturation effect. The aim of this study was to compare MBF determined by a traditional dual bolus method against a modified dual bolus approach and to evaluate both methods against PET in a porcine model of myocardial ischemia.
Methods:
Local myocardial ischemia was induced in five pigs, which were subsequently examined with contrast enhanced MRI (gadoteric acid) and PET (O-15 water). In the determination of MBF, the initial high concentration AIF was corrected using the ratio of low and high contrast AIF areas, normalized according to the corresponding heart rates. MBF was determined from the MRI, during stress and at rest, using the dual bolus and the modified dual bolus methods in 24 segments of the myocardium (total of 240 segments, five pigs in stress and rest). Due to image artifacts and technical problems 53% of the segments had to be rejected from further analyses. These two estimates were later compared against respective rest and stress PET-based MBF measurements.
Results:
Values of MBF were determined for 112/240 regions. Correlations for MBF between the modified dual bolus method and PET was rs = 0.84, and between the traditional dual bolus method and PET rs = 0.79. The intraclass correlation was very good (ICC = 0.85) between the modified dual bolus method and PET, but poor between the traditional dual bolus method and PET (ICC = 0.07).
Conclusions:
The modified dual bolus method showed a better agreement with PET than the traditional dual bolus method. The modified dual bolus method was found to be more reliable than the traditional dual bolus method, especially when there was variation in the heart rate. However, the difference between the MBF values estimated with either of the two MRI-based dual-bolus methods and those estimated with the gold-standard PET method were statistically significant.
Insights
A modified dual bolus MRI method improves myocardial blood flow (MBF) quantification accuracy compared to the traditional method, especially with heart rate variations. Both MRI methods showed statistically significant differences from PET measurements.
Area of Science:
- Cardiovascular Imaging
- Medical Physics
- Radiology
Background:
- Accurate myocardial blood flow (MBF) quantification using MRI requires correcting arterial input function (AIF) errors due to T1 saturation.
- Myocardial ischemia studies necessitate reliable MBF measurements.
Purpose of the Study:
- To compare MBF determined by a traditional dual bolus MRI method against a modified dual bolus approach.
- To evaluate both MRI methods against Positron Emission Tomography (PET) in a porcine model of myocardial ischemia.
Main Methods:
- Myocardial ischemia was induced in pigs, followed by contrast-enhanced MRI and PET scans.
- AIF correction was applied using the ratio of low and high contrast AIF areas, normalized for heart rate.
- MBF was calculated using both dual bolus MRI methods and compared against PET-derived MBF.
Main Results:
- The modified dual bolus MRI method demonstrated a strong correlation with PET (rs=0.84, ICC=0.85).
- The traditional dual bolus MRI method showed a weaker correlation with PET (rs=0.79, ICC=0.07).
- Despite improved agreement, statistically significant differences existed between MRI-derived and PET-derived MBF values.
Conclusions:
- The modified dual bolus method offers improved reliability for MBF quantification over the traditional method, particularly with heart rate variability.
- Further refinement is needed as statistically significant differences persist between MRI and PET MBF measurements.
Related Concept Videos
01:30Radiological Investigation II: MRI and Ventilation Perfusion Scan
Magnetic Resonance Imaging (MRI) and Ventilation Perfusion Scans are two radiological investigations that offer detailed diagnostic images of the body, particularly lung structures.
MRI
MRI uses magnetic fields and radiofrequency signals to distinguish between normal and abnormal tissues. This technology provides a more detailed diagnostic image than CT scans, enabling it to characterize pulmonary nodules, stage bronchogenic carcinoma, and evaluate inflammatory activity in...
01:14Imaging Studies I: CT and MRI
Description of the Procedures
Computed Tomography (CT) scan:
Computed Tomography (CT) scans use X-ray technology to generate detailed images of bones, organs, and tissues. During the scan, the patient lies on a moving table...
01:03Inertial Frames of Reference
01:27Non-inertial Frames of Reference
01:18Two-Compartment Open Model: IV Bolus Administration
The disparity between drug input and the sum of drug transfer rates between...
01:17Chemical Shift: Internal References and Solvent Effects
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...