Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Replication02:40

DNA Replication

58.8K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
58.8K
The DNA Replication Fork01:02

The DNA Replication Fork

40.6K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
40.6K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

16.6K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
16.6K
RNA Structure01:23

RNA Structure

78.9K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
78.9K
Replication in Prokaryotes02:35

Replication in Prokaryotes

97.2K
Overview
97.2K
From DNA to Protein03:06

From DNA to Protein

22.2K
The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
22.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dimension-controlled formation of crease patterns on soft solids.

Soft matter·2016
Same author

Modeling Day-to-day Flow Dynamics on Degradable Transport Network.

PloS one·2016
Same author

Tetramethylpyrazine Protects Against Glucocorticoid-Induced Apoptosis by Promoting Autophagy in Mesenchymal Stem Cells and Improves Bone Mass in Glucocorticoid-Induced Osteoporosis Rats.

Stem cells and development·2016
Same author

Corrigendum: Lithium-ion-based solid electrolyte tuning of the carrier density in graphene.

Scientific reports·2016
Same author

PTEN/PI3K/AKT protein expression is related to clinicopathological features and prognosis in breast cancer with axillary lymph node metastases.

Human pathology·2016
Same author

Comparing the Diagnostic Accuracy of RTE and SWE in Differentiating Malignant Thyroid Nodules from Benign Ones: a Meta-Analysis.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology·2016

Related Experiment Video

Updated: Jan 21, 2026

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

931

Human replication protein A induces dynamic changes in single-stranded DNA and RNA structures.

Qing-Man Wang1, Yan-Tao Yang1, Yi-Ran Wang1

  • 1State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.

The Journal of Biological Chemistry
|July 28, 2019
PubMed
Summary
This summary is machine-generated.

Replication protein A (RPA) dynamically binds to single-stranded DNA (ssDNA), altering its structure. This study reveals RPA

Keywords:
DNA binding proteinDNA recombinationDNA replicationDNA structureDNA-protein interactionenzyme mechanismfluorescence resonance energy transfer (FRET)molecular dynamicsreplication protein Asingle-molecule biophysics

More Related Videos

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
13:46

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography

Published on: September 29, 2011

32.3K
Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
06:25

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Published on: February 10, 2023

2.4K

Related Experiment Videos

Last Updated: Jan 21, 2026

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

931
Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
13:46

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography

Published on: September 29, 2011

32.3K
Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
06:25

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Published on: February 10, 2023

2.4K

Area of Science:

  • Molecular Biology
  • Genomics
  • Biophysics

Background:

  • Replication protein A (RPA) is crucial for eukaryotic genome maintenance.
  • RPA interacts with single-stranded DNA (ssDNA) via multiple binding modes.
  • The dynamic nature of RPA-ssDNA interactions and ssDNA structural modifications remain poorly understood.

Purpose of the Study:

  • To systematically investigate the dynamic interaction between human RPA and ssDNA using single-molecule Förster Resonance Energy Transfer (smFRET).
  • To elucidate how RPA binding influences ssDNA structure and how RPA transitions between different binding modes.
  • To explore RPA's interaction with RNA.

Main Methods:

  • Single-molecule Förster Resonance Energy Transfer (smFRET) to study RPA-ssDNA interactions.
  • Fluorescence anisotropy to assess RPA-RNA binding affinity.
  • Observation of RPA dynamics at the single-molecule level.

Main Results:

  • RPA forms distinct complexes with ssDNA, causing ssDNA to straighten or bend based on length, structure, and RPA concentration.
  • Observed both dynamic and relatively static RPA-ssDNA complexes.
  • RPA associates with RNA with lower affinity than ssDNA, exhibiting rapid, repetitive binding and dissociation from RNA.

Conclusions:

  • A model for RPA's dynamic engagement with ssDNA is proposed, highlighting structural modulation.
  • RPA exhibits differential binding dynamics with ssDNA and RNA.
  • Findings offer new insights into the complex dynamics of RPA interactions with nucleic acids.