Transforming compound leaf patterning by manipulating REVOLUTA in Medicago truncatula.
Chuanen Zhou1, Lu Han1, Yang Zhao1
1The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao, 266237, China.
The Plant Journal : for Cell and Molecular Biology
|July 28, 2019
Summary
Class III homeodomain-leucine zipper (HD-ZIPIII) genes regulate leaf polarity. A study in Medicago truncatula reveals REVOLUTA (MtREV1) is crucial for compound leaf development and auxin homeostasis.
Area of Science:
- Plant developmental biology
- Molecular genetics
- Plant physiology
Background:
- Leaves develop polarity along three axes: dorsoventral, proximodistal, and mediolateral.
- Class III homeodomain-leucine zipper (HD-ZIPIII) genes, regulated by microRNA165/166, are key for adaxial leaf identity.
- The specific roles of HD-ZIPIII genes in compound leaf development remain largely unknown.
Purpose of the Study:
- To investigate the function of HD-ZIPIII genes in compound leaf development in Medicago truncatula.
- To elucidate the role of REVOLUTA (MtREV1) in establishing leaf polarity and leaflet number.
- To understand the relationship between MtREV1, auxin homeostasis, and leaf patterning.
Main Methods:
- Screening of a retrotransposon-tagged mutant population in Medicago truncatula.
- Phenotypic characterization of a mutant with altered leaflet numbers.
- Genetic analysis of the REVOLUTA (MtREV1) gene and its role in leaf development.
- Analysis of auxin content in wild-type and mutant plants.
Main Results:
- A mutant with increased leaflet number and partial loss of adaxial identity was identified.
- A lesion in the HD-ZIPIII gene REVOLUTA (MtREV1) caused these developmental defects.
- Overexpression of an MtMIR166-insensitive MtREV1 resulted in adaxialized leaves and ectopic leaflets.
- Alterations in leaf patterning were associated with changes in free auxin content.
Conclusions:
- MtREV1 is essential for determining leaf adaxial-abaxial polarity in Medicago truncatula.
- MtREV1 plays a critical role in the patterning of compound leaves, influencing leaflet number.
- The function of MtREV1 in leaf development is linked to maintaining proper auxin homeostasis.
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