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Calcium-binding protein in human duodenal biopsies
M Staun1, S Boesby, H Daugaard
1Department of Medicine P, Rigshospitalet, Copenhagen, Denmark.
Calcified Tissue International
|April 1, 1988
Summary
Intestinal calcium-binding protein (CaBP) levels vary widely in humans but do not change with age. CaBP concentration significantly correlates with serum 1,25-dihydroxyvitamin D levels.
Area of Science:
- Biochemistry
- Gastroenterology
- Endocrinology
Background:
- Intestinal calcium-binding protein (CaBP) plays a crucial role in calcium absorption.
- Understanding CaBP levels in the duodenum is important for gastrointestinal and metabolic research.
Purpose of the Study:
- To quantify intestinal CaBP levels in adults with dyspepsia.
- To investigate the relationship between CaBP, age, and vitamin D status.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) was used to measure CaBP in duodenal biopsies.
- 94 patients with dyspepsia and no gastroduodenoscopy findings were analyzed.
- Correlations with age, serum 1,25-dihydroxyvitamin D, parathyroid hormone, and alkaline phosphatase were assessed.
Main Results:
- Median duodenal CaBP was 6.0 µg/mg cytosolic protein, with high inter-individual variation.
- No significant correlation was found between CaBP levels and patient age.
- A strong positive correlation (P < 0.001) was observed between CaBP concentration and serum 1,25-dihydroxyvitamin D.
Conclusions:
- Human duodenal CaBP exhibits significant variability and is independent of age.
- Serum 1,25-dihydroxyvitamin D is a key regulator of intestinal CaBP expression in vivo.