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The effect of hydroxyapatite crystallinity on hemolysis.

J Wiessner¹, G Mandel, P Halverson

¹Clement J. Zablocki Veterans Administration Medical Center, Milwaukee 53295.

Calcified Tissue International

|April 1, 1988

Summary

Crystalline hydroxyapatite

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Area of Science:

Biomineralization and Materials Science
Cell Biology and Toxicology

Background:

Crystalline hydroxyapatite is a key component in biological tissues and pathological calcifications.
Apatite's crystal structure allows for diverse atomic substitutions, leading to varied biochemical and physical properties.
Apatite crystallites interact with cellular systems, potentially altering cellular function.

Purpose of the Study:

To investigate the relationship between apatite characteristics and their interaction with cell membranes.
To quantify crystal-induced membranolysis of human red blood cells by different apatite preparations.

Main Methods:

Measured hemolytic potentials of 29 apatite preparations at constant crystal surface areas over time.
Characterized each apatite sample by morphology, particle size, carbonate content, zeta potential, and X-ray diffraction line broadening.
Analyzed correlations between physical-chemical properties and hemolytic potential.

Main Results:

Hemolytic potential was inversely correlated with surface area per gram and X-ray diffraction line broadening.
These two parameters indicate the degree of crystallinity of the apatite samples.
Other tested parameters (morphology, particle size, % CO3, zeta potential) did not show significant correlation.

Conclusions:

The degree of crystallinity, reflected by surface area and X-ray diffraction line broadening, is a critical factor in hydroxyapatite's interaction with red blood cell membranes.
Understanding these interactions is crucial for predicting the biological effects of hydroxyapatite in various applications and pathological conditions.

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