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Updated: Jan 21, 2026

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells
Published on: February 8, 2018
Dok-3 deficient mice display different immune clustering and Tim-3 expression
Wenjiang Yan1,2, Yijia Tian3, Peng Sun1,4
1The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, and The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, No. 107 Wenhua Xi Road, Jinan, Shandong, China.
Background:
Dok-3 has been shown to play an important role in immune system. Tim-3 also has been recognized as an important immune regulator which involves in many diseases. The relationship of them is still unclear.
Methods:
We detected the expression of Tim-3 on spleen immune cells from Dok-3 deficient mice and control mice by flow cytometry.
Results:
In this article, we found that Dok-3-/- mice display almost entirely different immune clustering characteristics compared with wild type 129 mice. The CD4 T cells and CD8 T cells decreased and DC cells, macrophages, MDSCs increased when the Dok-3 gene knocked-out. The Tim-3 expression on CD4 T cells, CD8 T cells, NK cells, DC cells increased when the Dok-3 gene knocked-out. The macrophages and MDSCs just display the opposite results.
Conclusions:
Although Dok-3-/- mice display different immune clustering and Tim-3 expression, the mechanism still needs further study.
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