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Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells
Published on: February 8, 2018
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Dok-3 deficient mice display different immune clustering and Tim-3 expression
Wenjiang Yan1,2, Yijia Tian3, Peng Sun1,4
1The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, and The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, No. 107 Wenhua Xi Road, Jinan, Shandong, China.
European Journal of Medical Research
|July 29, 2019
Summary
Dok-3 deficiency significantly alters immune cell populations and Tim-3 expression in mice. These findings highlight Dok-3
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- Dok-3 plays a crucial role in immune system regulation.
- Tim-3 is recognized as a key immune regulator implicated in various diseases.
- The interplay between Dok-3 and Tim-3 remains largely undefined.
Purpose of the Study:
- To investigate the impact of Dok-3 deficiency on immune cell clustering.
- To determine the effect of Dok-3 knockout on Tim-3 expression in splenic immune cells.
Main Methods:
- Flow cytometry was employed to analyze spleen immune cells.
- Comparison was made between Dok-3 deficient (Dok-3-/-) mice and wild-type control mice.
Main Results:
- Dok-3 knockout mice exhibited distinct immune cell profiles compared to wild-type controls.
- A decrease in CD4 T cells and CD8 T cells, alongside an increase in DC cells, macrophages, and MDSCs, was observed in Dok-3-/- mice.
- Tim-3 expression was upregulated on CD4 T cells, CD8 T cells, NK cells, and DC cells in Dok-3-/- mice, while macrophages and MDSCs showed the opposite trend.
Conclusions:
- Dok-3 deficiency profoundly influences immune cell distribution and Tim-3 expression patterns.
- The underlying mechanisms driving these observed changes warrant further in-depth investigation.
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