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MicroRNA Expression Profiles in External Cervical Resorption

Mary T Pettiette1, Shaoping Zhang2, Antonio J Moretti3

  • 1Department of Endodontics, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.

Journal of Endodontics
|July 29, 2019
PubMed
Abstract

Insights

External cervical resorption (ECR) has unique microRNA (miRNA) expression profiles compared to chronic periodontitis. These differentially expressed miRNAs target genes involved in inflammation, immunity, and mineralized tissue metabolism.

Area of Science:

  • Oral Biology
  • Molecular Biology
  • Biochemistry

Background:

  • External cervical resorption (ECR) presents diagnostic and therapeutic challenges due to unknown etiology and pathogenesis.
  • MicroRNA (miRNA) expression patterns in ECR lesions remain largely uncharacterized.

Purpose of the Study:

  • To characterize miRNA expression in human ECR tissues.
  • To identify potential messenger RNA (mRNA) targets and associated biological pathways for ECR.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) array analysis of 88 miRNAs in ECR, chronic periodontitis (CP), and control tissues.
  • Bioinformatic analyses (miRWalk, KEGG pathways) to predict mRNA targets and signaling pathways.
  • Statistical analysis (Student t test) to identify differentially expressed miRNAs.

Main Results:

  • Three miRNAs (miR-20a-5p, miR-210-3p, miR-99a-4p) were downregulated, and one (miR-122-5p) was upregulated in ECR.
  • ECR and CP exhibited distinct miRNA expression profiles, with 3 differentially expressed miRNAs identified between them.
  • Predicted pathways modulated by miRNAs in ECR include glycosaminoglycan biosynthesis, mineral absorption, and insulin signaling.

Conclusions:

  • ECR exhibits unique miRNA expression profiles compared to CP, suggesting distinct pathobiology.
  • Differentially expressed miRNAs in ECR are predicted to target genes regulating inflammation, immunity, and mineralized tissue metabolism.
  • This study provides a foundation for understanding the molecular mechanisms underlying ECR.
Keywords:
Chronic periodontitisexternal cervical resorptioninflammationmicroRNA

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