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Updated: Jan 21, 2026

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
Published on: April 17, 2017
Protein dynamics and conformational changes explored by hydrogen/deuterium exchange mass spectrometry
Jie Zheng1, Timothy Strutzenberg1, Bruce D Pascal2
1The Scripps Research Institute, Department of Molecular Medicine, Jupiter, FL 33458, USA; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, Jupiter, FL 33458, USA.
Abstract:
Proteins are not rigid bodies under their physiological conditions. Here we discuss a solution-phase structural proteomics technique, hydrogen deuterium exchange coupled with mass spectrometry (HDX-MS), as a means to study protein dynamics, which can complement other structural approaches. We outline the background theory and highlight the utility of HDX-MS measurements in two case studies involving a nuclear receptor and an innate immunity receptor. We also discuss emerging software advances for improving data analysis and three-dimensional visualization.
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