Related Experiment Video
Updated: Jan 21, 2026

Denaturing Gradient Gel Electrophoresis DGGE
Published on: February 25, 2007
Gel electrophoresis of a soft particle
1Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki Noda, Chiba 278-8510, Japan.
Abstract:
A review is given on the theory of the gel electrophoresis of a spherical soft particle, i.e., the electrophoresis of a spherical hard particle covered with an ion-penetrable surface layer of polyelectrolytes moving in a polymer gel medium, which may either be charged or uncharged. A detailed study is made on the fundamental electrokinetic equations describing the soft-particle gel electrophoresis due to the long-range hydrodynamic particle-gel interaction on the basis of the Brinkman-Debye-Bueche model. A general expression and several approximate analytic expressions are given for the electrophoretic mobility of a soft particle in a polymer gel medium.
Related Concept Videos
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
DNA Agarose Gel Electrophoresis
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Subatomic Particles
Electrophoresis: Overview
There...
The Nucleosome Core Particle
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
The Nucleosome Core Particle
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...

