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Effect of temperature on alpha-amylase formation and DNA replication in Bacillus subtilis
J Hao1, J Pazlarová, M Strnadová
1Department of Enzyme Engineering, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
Folia Microbiologica
|January 1, 1988
Abstract:
The effect of temperature on extracellular alpha-amylase synthesis and chromosomal and plasmid DNA replication in Bacillus subtilis A18 carrying plasmid pMI10 was studied. The specific growth rate mu increased with elevated temperature up to 42.5 degrees C, while the activities of alpha-amylase per population dry mass decreased. No obvious quantitative changes of 14C-thymidine incorporation per dry mass increase and no basic differences in plasmid copy number in the range of temperatures from 25 to 40 degrees C were found.