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Updated: Jul 28, 2026
15:42
Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers
Published on: March 6, 2009
Activated T cells and monocytes have characteristic patterns of class II antigen expression
P A Robbins1, V C Maino, N L Warner
1Becton Dickinson Immunocytometry Systems, Mountain View, CA 94043.
Journal of Immunology (Baltimore, Md. : 1950)
|August 15, 1988
Summary
Activated T cells and monocytes express human histocompatibility class II antigens. T cell activation by anti-CD3 induces class II antigen expression, potentially via a T cell-derived lymphokine.
Area of Science:
- Immunology
- Cell Biology
Background:
- Human histocompatibility class II antigens (HLA-DR, -DQ, -DP) are crucial for T cell recognition.
- Understanding HLA class II expression on immune cells like T cells and monocytes is vital for immune response studies.
Purpose of the Study:
- To quantify human histocompatibility class II antigen expression on activated T cells and monocytes.
- To analyze the factors influencing class II antigen expression during T cell activation.
Main Methods:
- Quantitative monoclonal antibody (mAb) binding assays.
- Direct two-color immunofluorescence.
- Flow cytometry to measure antigen expression on T cells and monocytes.
Main Results:
- Activated monocytes express significantly higher levels of HLA class II antigens (DR, DQ, DP) compared to resting cells.
- A subpopulation of T cells activated by anti-CD3 also expresses HLA class II antigens.
- T cell activation conditions, including anti-CD3 concentration and initial monocyte levels, influence class II antigen expression.
Conclusions:
- Activated T cells can express human histocompatibility class II antigens.
- A lymphokine produced by CD3-activated T cells may induce class II antigen expression on T cells.
- Further research is needed to elucidate the specific lymphokine and its mechanism of action.
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