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In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
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MicroRNA-153 Decreases Tryptophan Catabolism and Inhibits Angiogenesis in Bladder Cancer by Targeting Indoleamine
Wentao Zhang1,2, Shiyu Mao1, Donghui Shi3
1Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.
Frontiers in Oncology
|July 30, 2019
Summary
MicroRNA-153 (miR-153) is downregulated in bladder cancer, inhibiting tumor growth and metastasis by targeting indoleamine 2,3-dioxygenase 1 (IDO1). This suggests miR-153 is a potential therapeutic target for bladder cancer.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Metastasis is a leading cause of cancer mortality.
- Investigating molecular mechanisms of bladder cancer is crucial.
- Understanding microRNA roles in cancer progression is vital.
Purpose of the Study:
- To assess microRNA-153 (miR-153) expression in bladder cancer.
- To elucidate the molecular mechanism of miR-153 in bladder cancer.
- To evaluate miR-153 as a potential therapeutic target.
Main Methods:
- qRT-PCR and TCGA dataset analysis for miR-153 expression and prognosis.
- In vitro (cell lines) and in vivo (nude mouse xenograft, CAM assays) experiments.
- Luciferase reporter assay to confirm miR-153 targets (IDO1) and signaling pathways (IL6/STAT3/VEGF).
Main Results:
- miR-153 expression is downregulated in bladder cancer, correlating with advanced stage and poor survival.
- miR-153 inhibits bladder cancer cell growth, apoptosis, migration, invasion, and EMT.
- miR-153 suppresses angiogenesis and targets IDO1, impacting tryptophan metabolism via IL6/STAT3/VEGF signaling.
Conclusions:
- miR-153 exhibits anti-tumor activity in bladder cancer by targeting IDO1.
- miR-153 suppresses tumor growth, metastasis, and angiogenesis.
- miR-153 represents a promising therapeutic target for bladder cancer.
![Radiosynthesis of 1-2-[18F]Fluoroethyl-L-Tryptophan using a One-pot, Two-step Protocol](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F63025.jpg&w=3840&q=50)
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