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Updated: Jan 21, 2026

Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks
Published on: November 3, 2017
53BP1-DNA repair enters a new liquid phase
Rossana Piccinno1, Vera Minneker1, Vassilis Roukos1
1Institute of Molecular Biology (IMB), Mainz, Germany.
Abstract:
In response to DNA damage, transient repair compartments in the nucleus concentrate repair proteins and activate downstream signaling factors. In this issue of The EMBO Journal, Kilic et al show that DNA repair focal assemblies marked by accumulation of 53BP1 are phase separated liquid compartments. This liquid droplet-like behavior of 53BP1 compartments might help to coordinate local lesion recognition with global gene activation in response to DNA damage.
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