Pseudomonas huaxiensis sp. nov., isolated from hospital sewage

A novel Gram-negative, obligate aerobic, rod-shaped, motile by one or two polar flagella, non-spore-forming bacterial strain, WCHPs060044^T, was isolated from the wastewater treatment plant at West China Hospital in Chengdu, PR China. Analysis of its 16S rRNA gene sequence showed that strain WCHPs060044^T belonged to the genus Pseudomonas with the highest similarity to Pseudomonas alkylphenolica KL28^T (99.73 %), Pseudomonas donghuensis HYS^T (99.52 %) and Pseudomonas wadenswilerensis CCOS 846^T (99.38 %). Phylogenomic analysis based on 107 core gene sequences demonstrated that WCHPs060044^T was a member of the Pseudomonas putida group but was distant from all closely related species. Whole-genome comparisons, using average nucleotide identity based on blast (ANIb) and in silico DNA-DNA hybridization (isDDH), confirmed low genome relatedness to its close phylogenetic neighbours [below the recommended thresholds of 95 % (ANIb) and 70 % (isDDH) for species delineation]. Phenotypic characterization tests showed that the utilization of l-arginine, l-fucose, d-galacturonic, d-glucuronic acid, inositol and sorbitol in combination could distinguish this strain from other related species of the genus Pseudomonas. Therefore, based on genetic and phenotypic evidence, strain WCHPs060044^T represents a novel species, for which the name Pseudomonas huaxiensis sp. nov. is proposed. The type strain is WCHPs060044^T (GDMCC 1.1396^T=JCM 32907^T=CCTCC AB 2018120^T=CNCTC 7663^T).