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Identification and Dissection of Diverse Mouse Adipose Depots
Published on: July 11, 2019
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Identification and Dissection of Diverse Mouse Adipose Depots
Devika P Bagchi1, Ormond A MacDougald2
1Department of Molecular & Integrative Physiology, University of Michigan Medical School; dpbagchi@med.umich.edu.
Journal of Visualized Experiments : Jove
|July 30, 2019
Summary
This study provides a mouse dissection guide for identifying distinct adipose tissue depots. Standardized methods enable comparisons of fat tissue characteristics and their role in metabolic diseases.
Area of Science:
- Physiology
- Anatomy
- Metabolism
Background:
- Adipose tissues are crucial for energy storage, heat generation, and immune regulation.
- Regional differences in fat depots impact metabolic health and disease.
- Understanding these depot-specific properties is vital for metabolic disease research.
Purpose of the Study:
- To provide a detailed anatomic atlas and dissection protocol for mouse adipose tissues.
- To enable reproducible identification and excision of diverse fat depots.
- To facilitate comparative studies on the molecular and metabolic functions of different adipose depots.
Main Methods:
- Development of a detailed anatomic atlas for mouse adipose tissues.
- Standardized dissection guide for reproducible identification and excision of fat depots.
- Protocol designed for comparative analysis of molecular and metabolic characteristics.
Main Results:
- The protocol ensures accurate identification and excision of various mouse adipose depots.
- Standardized dissection allows for reliable comparisons between different fat tissues.
- Enables investigation into depot-specific contributions to local and systemic pathologies.
Conclusions:
- This protocol standardizes adipose tissue dissection in mice.
- Facilitates research into depot-specific functions and metabolic disease mechanisms.
- Aids in understanding the role of distinct fat depots in whole-body homeostasis.
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