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Development of an in vitro model to study tooth cystogenesis
N K Laureano1, L Bernardi1,2, L Bundrich1
1Basic Research Center, Dentistry School, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
International Endodontic Journal
|July 30, 2019
Summary
This study presents a novel in vitro model for studying radicular cyst development. The experimental model successfully created cystic structures from epithelial cells, offering a new tool for research.
Area of Science:
- Oral pathology
- Cell biology
- Biomaterials science
Background:
- Radicular cysts are common inflammatory jaw cysts.
- Understanding their development is crucial for effective treatment.
- Current research models have limitations in mimicking in vivo conditions.
Purpose of the Study:
- To develop and validate an in vitro experimental model for studying radicular cyst formation.
- To investigate the feasibility of using spheroid cultures in a 3D collagen matrix for cystogenesis research.
Main Methods:
- Epithelial cells (HaCaT, Cal27) were cultured as spheroids in agarose-coated plates.
- Spheroids were embedded in a 3D collagen matrix and cultured for up to 21 days.
- Histological and confocal microscopy were used to analyze spheroid morphology and cyst formation.
Main Results:
- Both cell lines formed stable spheroids within 24 hours.
- Histological analysis revealed cavity formation with epithelial lining resembling radicular cysts by day 3.
- HaCaT spheroids demonstrated greater stability in the collagen matrix compared to Cal27.
Conclusions:
- An in vitro model for radicular cystogenesis was successfully established.
- This model allows for the investigation of cyst development mechanisms.
- The model can be utilized to test therapeutic interventions and study growth factor effects.
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