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Related Concept Videos

Genetic Screens02:46

Genetic Screens

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Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
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Types of Fever01:25

Types of Fever

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Fever can be triggered by several factors, including infections, nervous system disorders, certain cancers, blood diseases like leukemia, embolism, thrombosis, heatstroke, dehydration, surgical trauma, crushing injuries, and allergic reactions.
Here are the different types of fever:
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Patterns of Fever01:26

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Before understanding the types and patterns of fever, it is essential to know its phases.
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Fixing Double-strand Breaks02:04

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The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
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Lagging Strand Synthesis01:59

Lagging Strand Synthesis

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During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
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What are Viruses?00:50

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Related Experiment Video

Updated: Jan 21, 2026

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
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Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

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High-throughput screening for negative-stranded hemorrhagic fever viruses using reverse genetics.

Lisa Wendt1, Linus Bostedt2, Thomas Hoenen1

  • 1Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Greifswald, Insel Riems, Germany.

Antiviral Research
|July 30, 2019
PubMed
Summary
This summary is machine-generated.

Developing new treatments for viral hemorrhagic fevers (VHFs) is challenging. This review highlights reverse genetics systems for identifying broad-acting antivirals and understanding virus-host interactions for future therapies.

Keywords:
(3–10 max.): reverse geneticsAntiviral screeningHigh-throughput screeningLife cycle modellingMinigenome systemsViral hemorrhagic fever

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Area of Science:

  • Virology
  • Molecular Biology
  • Drug Discovery

Background:

  • Viral hemorrhagic fevers (VHFs) cause significant global mortality annually.
  • Limited treatment options exist due to the low commercial viability of single-target drugs.
  • Drug repurposing and broad-spectrum therapies targeting host responses are attractive strategies.

Purpose of the Study:

  • To review available reverse genetics systems for negative-sense VHF viruses.
  • To highlight advancements in applying these systems for high-throughput screening (HTS).
  • To identify potential antivirals and novel virus-host interactions for future VHF treatments.

Main Methods:

  • Utilizing reverse genetics-based approaches, including full-length clone (FLC) systems.
  • Employing life cycle modeling (LCM) systems to study specific viral replication steps.
  • Applying these systems for high-throughput screening (HTS) to discover therapeutic targets.

Main Results:

  • Reverse genetics systems, particularly LCM, facilitate targeted screening and mechanism of action studies.
  • Recent advancements have improved the application of these systems for HTS.
  • These tools aid in identifying potential broad-range antivirals and critical virus-host interactions.

Conclusions:

  • Reverse genetics systems are crucial for advancing VHF research and drug discovery.
  • LCM systems offer valuable insights into viral replication and potential therapeutic interventions.
  • This review provides a foundation for developing new treatments against deadly VHF-causing viruses.