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Updated: Jan 21, 2026
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Super-resolution Imaging of Neuronal Dense-core Vesicles
Published on: July 2, 2014
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Modulation and study of photoblinking behavior in dye doped silver-silica core-shell nanoparticles for localization
Chumki Chakraborty1, S Thompson2, Veronica J Lyons2
1Department of Mechanical Engineering, Texas Tech University, Lubbock, TX 79409, United States of America.
Nanotechnology
|July 30, 2019
Summary
Controlling fluorescent nanoparticle blinking is key for advanced imaging. Researchers found nitrogen-rich conditions and nanoparticle hydration significantly impact blinking behavior in silver core silica nanoparticles.
Area of Science:
- Nanotechnology
- Materials Science
- Optical Physics
Background:
- Fluorescent nanoparticle blinking is a critical phenomenon in imaging.
- Understanding and controlling blinking is vital for applications in optical, semiconductor, and fluorescent imaging.
- Self-blinking nanomaterials are valuable for super-resolution microscopy.
Purpose of the Study:
- To synthesize silver core silica nanoparticles (Ag@SiO2) doped with Rhodamine 110.
- To investigate parameters influencing the blinking behavior of these nanoparticles.
- To confirm single-particle emission during blinking events.
Main Methods:
- Synthesis of Ag@SiO2 nanoparticles doped with Rhodamine 110.
- Controlled exposure to nitrogen-rich conditions.
- Comparative study of hydrated versus dried nanoparticles.
- Super-resolution microscopy and intensity histogram analysis.
Main Results:
- Nitrogen-rich conditions shifted nanoparticles towards higher duty cycles.
- Hydrated nanoparticles exhibited a less pronounced response to nitrogen-rich conditions compared to dried ones.
- Surrounding matrix influences nanoparticle response to molecular oxygen.
- Blinking confirmed as a single-particle phenomenon, not multi-body.
Conclusions:
- Blinking behavior of Ag@SiO2 nanoparticles is tunable via environmental conditions.
- The surrounding matrix plays a significant role in modulating blinking.
- Single-particle emission is confirmed, validating their use in super-resolution microscopy.
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