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Updated: Jan 21, 2026
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Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples
Published on: December 2, 2022
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Target-catalyzed hairpin structure-mediated padlock cyclization for ultrasensitive rolling circle amplification
Huanxia Song1, Zhe Yang2, Min Jiang1
1Zhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
Talanta
|July 31, 2019
Summary
A new ultrasensitive rolling circle amplification (THP-RCA) method detects the STAT3 gene, a cancer-promoting gene. This technique shows promise for early tumor diagnosis and targeted cancer therapies.
Area of Science:
- Biochemistry
- Molecular Biology
- Oncology
Background:
- STAT3 is a proto-oncogene crucial in tumor development.
- Accurate detection of STAT3 is vital for cancer diagnosis and therapy.
Purpose of the Study:
- To develop a novel, ultrasensitive method for detecting the STAT3 gene.
- To establish a homogenous solution-based assay for proto-oncogene detection.
Main Methods:
- Utilized target-catalyzed hairpin structure-mediated padlock cyclization (THP-RCA).
- Employed HP1 as a template/primer and HP2 as a padlock probe.
- Generated amplified single-stranded DNA products for fluorescence detection via molecular beacons.
Main Results:
- Achieved ultrasensitive detection of the STAT3 gene down to 100 fM.
- Demonstrated high specificity for wild-type STAT3 DNA.
- Validated the system's effectiveness in human serum samples.
Conclusions:
- The THP-RCA strategy offers a highly sensitive and specific method for STAT3 detection.
- This approach holds significant potential for clinical applications in tumor diagnosis and treatment guidance.
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