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Updated: Jul 1, 2026

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS
Published on: April 13, 2016
Determination of Zn, Cu and Fe in human patients' serum using micro-sampling ICP-MS and sample dilution
Tharwat N Abduljabbar1, Barry L Sharp2, Helen J Reid2
1Department of Chemistry, Faculty of Science, King Abdul-Aziz University, P.O. Box 80200, Jeddah 21589, Saudi Arabia; Centre for Analytical Science, Department of Chemistry, Loughborough University, Loughborough, Leicestershire, LE11 3TU, United Kingdom.
Abstract:
A high-throughput, sensitive and rapid method was developed for the determination of Zn, Cu and Fe in small volumes (30 μL) of human serum using inductively coupled plasma mass spectrometry (ICP-MS). The sample preparation procedure employed simple 100-fold dilution of the serum samples with 1.0% butanol, 0.5% v/v ammonia, 0.02% v/v Triton X-100 and 0.01% v/v HNO3. The reliability of the method was evaluated using serum UTAK certified reference material, and the results matched well with the certified values. The method was applied to determine Zn, Cu and Fe in 81 human serum samples from participants in Alzheimer disease (AD) and age-related macular degeneration (AMD) studies. No significant differences were found in Zn and Cu levels between age matched controls, AD and AMD patients. Whilst iron levels appeared marginally higher in the AMD group, compared with the AD group, iron showed larger overall variability than the other two elements.
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