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Related Experiment Video

Updated: Jan 21, 2026

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles

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Published on: January 7, 2019

Prolonged Release and Functionality of Interleukin-10 Encapsulated within PLA-PEG Nanoparticles.

Skyla A Duncan¹, Saurabh Dixit¹, Rajnish Sahu¹

¹Center for NanoBiotechnology & Life Sciences Research, Department of Biological Sciences, Alabama State University, 915 South Jackson Street, Montgomery, AL 36104, USA.

Nanomaterials (Basel, Switzerland)

|July 31, 2019

Summary

We encapsulated anti-inflammatory interleukin-10 (IL-10) into nanoparticles to extend its therapeutic half-life. This novel approach shows potential for treating chlamydial infections and other inflammatory diseases.

Keywords:

IL-10 PLA-PEG nanoparticles SOCS1 SOCS3 anti-inflammatory chlamydia cytokines inflammation recombinant major outer membrane protein (rMOMP)therapy

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Last Updated: Jan 21, 2026

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Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier

Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier

Published on: February 8, 2017

Area of Science:

Biomedical Engineering
Immunology
Nanotechnology

Background:

Inflammation is key in chlamydial infections, with interleukin-10 (IL-10) showing anti-inflammatory properties.
Clinical use of IL-10 is limited by its short half-life.

Purpose of the Study:

To prolong the half-life of IL-10 by encapsulating it in Poly (lactic acid)-Poly (ethylene glycol) (PLA-PEG) nanoparticles.
To evaluate the characteristics and efficacy of IL-10 loaded nanoparticles.

Main Methods:

IL-10 was encapsulated into PLA-PEG nanoparticles.
Nanoparticle characterization included size, zeta potential, and polydispersity index.
In vitro release studies, thermal stability analysis (differential scanning calorimetry), and macrophage assays were performed.

Main Results:

Encapsulated IL-10 nanoparticles exhibited a size of ~238 nm, zeta potential of -14.2 mV, and encapsulation efficiency of ~77%.
A sustained release of IL-10 was observed for up to 60 days with favorable thermal stability up to 89 °C.
Encapsulated IL-10 modulated inflammatory cytokine release (IL-6, IL-12p40) and induced SOCS1/SOCS3 in macrophages.

Conclusions:

PLA-PEG nanoparticles successfully prolonged IL-10 half-life and maintained its bioactivity.
Encapsulated IL-10 demonstrates significant potential for treating chlamydial inflammatory diseases and other biomedical applications.