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Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles
Published on: January 7, 2019
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Prolonged Release and Functionality of Interleukin-10 Encapsulated within PLA-PEG Nanoparticles
Skyla A Duncan1, Saurabh Dixit1, Rajnish Sahu1
1Center for NanoBiotechnology & Life Sciences Research, Department of Biological Sciences, Alabama State University, 915 South Jackson Street, Montgomery, AL 36104, USA.
Nanomaterials (Basel, Switzerland)
|July 31, 2019
Summary
We encapsulated anti-inflammatory interleukin-10 (IL-10) into nanoparticles to extend its therapeutic half-life. This novel approach shows potential for treating chlamydial infections and other inflammatory diseases.
Area of Science:
- Biomedical Engineering
- Immunology
- Nanotechnology
Background:
- Inflammation is key in chlamydial infections, with interleukin-10 (IL-10) showing anti-inflammatory properties.
- Clinical use of IL-10 is limited by its short half-life.
Purpose of the Study:
- To prolong the half-life of IL-10 by encapsulating it in Poly (lactic acid)-Poly (ethylene glycol) (PLA-PEG) nanoparticles.
- To evaluate the characteristics and efficacy of IL-10 loaded nanoparticles.
Main Methods:
- IL-10 was encapsulated into PLA-PEG nanoparticles.
- Nanoparticle characterization included size, zeta potential, and polydispersity index.
- In vitro release studies, thermal stability analysis (differential scanning calorimetry), and macrophage assays were performed.
Main Results:
- Encapsulated IL-10 nanoparticles exhibited a size of ~238 nm, zeta potential of -14.2 mV, and encapsulation efficiency of ~77%.
- A sustained release of IL-10 was observed for up to 60 days with favorable thermal stability up to 89 °C.
- Encapsulated IL-10 modulated inflammatory cytokine release (IL-6, IL-12p40) and induced SOCS1/SOCS3 in macrophages.
Conclusions:
- PLA-PEG nanoparticles successfully prolonged IL-10 half-life and maintained its bioactivity.
- Encapsulated IL-10 demonstrates significant potential for treating chlamydial inflammatory diseases and other biomedical applications.
Keywords:
IL-10PLA-PEG nanoparticlesSOCS1SOCS3anti-inflammatorychlamydiacytokinesinflammationrecombinant major outer membrane protein (rMOMP)therapyMore Related Videos
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