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Updated: Jan 21, 2026

Using Mycobacterium smegmatis as a Bioindicator for Zinc-Limited Growth Conditions in Mycobacteria
Published on: September 20, 2024
INNO-LiPA DNA line probe assay misidentification of M. smegmatis as Mycobacterium fortuitum complex
Theo van den Broek1, Nard G Janssen2, David J Hetem3
1Department of Medical Microbiology, University Medical Center Utrecht, 3508, GA, Utrecht, The Netherlands.
Abstract:
Seven weeks after being kicked in the face by a cow, a 34-year-old male patient developed a posttraumatic mycobacterial lymphadenitis. A rapidly growing mycobacterial isolate cultured from a surgically drained lymphadenitis pus specimen was identified as Mycobacterium smegmatis by matrix-assisted laser desorption/ionization mass spectrometry and a combination of ITS-, hsp65-, and 16S rRNA-DNA sequence analysis, but as Mycobacterium fortuitum complex using the commercial INNO-LiPA Mycobacteria v2 line probe assay. As it is unclear if the misidentification of this strain is an exception, more research is required.
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