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Related Concept Videos

¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

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The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
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¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

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The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
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Applications Of NMR In Biology01:25

Applications Of NMR In Biology

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Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
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¹H NMR: Pople Notation01:09

¹H NMR: Pople Notation

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The Pople nomenclature system classifies spin systems based on the difference between their chemical shifts. Coupled spins are denoted by capital letters with subscripts indicating the number of equivalent nuclei. When the coupled nuclei have well-separated chemical shifts, they are assigned letters that are far apart in the alphabet, such as A and X. When the difference in chemical shifts is small, coupled nuclei are named using adjacent letters of the alphabet (AB, MN, or XY).
A proton...
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NMR Spectroscopy Of Amines01:19

NMR Spectroscopy Of Amines

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In proton NMR spectroscopy, primary amines and secondary amines showcase their N–H protons as a broad signal in the chemical shift range between δ 0.5 and 5 ppm. The exact position in this range depends on several factors, including sample concentration, hydrogen bonding, and the type of solvent used. Since amine protons undergo fast proton exchange in solution, the protons are labile and therefore do not participate in any splitting with adjacent protons. Thus, the observed peak is...
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NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

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NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
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Application and Methodology of the Non-destructive 19F Time-domain NMR Technique to Measure the Content in Fluorine-containing Drug Products
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Cells, drugs and NMR.

Gregg Siegal1, Philipp Selenko2

  • 1ZoBio B.V., BioPartner 2 Building, J.H. Oortweg 19, 2333 Leiden, the Netherlands.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|July 31, 2019
PubMed
Summary

High-resolution in-cell Nuclear Magnetic Resonance (NMR) spectroscopy offers new insights into cellular structures. Advancements in NMR technology are enhancing its role in cell-based drug discovery for academia and industry.

Keywords:
Dynamic nuclear polarizationIn vivo NMRIn-cell NMRMetabolomicsOn-cell NMRSolid-state NMRWhole-cell NMR

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Analytical Chemistry

Background:

  • Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful technique for analyzing cellular components.
  • Traditional in vivo and whole-cell NMR methods have been used for decades.
  • High-resolution in-cell NMR spectroscopy represents a significant advancement in cellular analysis.

Purpose of the Study:

  • To review the application of cellular NMR methods in drug discovery.
  • To highlight recent technological advancements in NMR for cellular applications.
  • To discuss the impact of these advancements on academic and industrial drug discovery.

Main Methods:

  • Review of existing literature on cellular NMR spectroscopy.
  • Discussion of technological developments including miniaturized bioreactors and flow-probe perfusion systems.
  • Analysis of NMR's contribution to cell-based drug discovery.

Main Results:

  • Technological progress has improved the robustness, reproducibility, and physiological relevance of cellular NMR.
  • Miniaturized bioreactors and flow-probe perfusion systems are key innovations.
  • NMR is increasingly consolidated as a vital tool in cell-based drug discovery.

Conclusions:

  • Cellular NMR spectroscopy, particularly high-resolution in-cell NMR, is a valuable method for drug discovery.
  • Technological advancements are crucial for expanding the utility of NMR in biological research.
  • NMR plays a consolidating role in both academic and industrial drug discovery pipelines.