Morphological Cell Types Projecting from V1 Layer 4B to V2 Thick and Thin Stripes

In macaque visual cortex, different cytochrome oxidase stripes of area V2 receive segregated projections from layers (L)2/3 and 4B of the primary visual cortex (V1), and project to dorsal or ventral stream extrastriate areas. Parallel V1-to-V2 pathways suggest functionally specialized circuits, but it is unknown whether these circuits arise from distinct cell types. V1 L4B includes two morphological types of excitatory projection neurons: pyramids, which carry mixed magnocellular (M) and parvocellular (P) information to downstream areas, and spiny stellates, which carry only M information. Previous studies have shown that, overall, V2 receives ∼80% of its L4B inputs from pyramids, thus receiving mixed M and P signals. However, it is unknown how pyramids and stellates distribute their outputs to the different V2 stripes, and whether different stripes receive inputs from morphologically distinct neuron types. Using viral-mediated labeling of V2-projecting L4B neurons in male macaques, we show that thick stripes receive a greater contribution of L4B inputs from M-dominated spiny stellates compared with thin stripes. Both stripe types, however, receive a much larger contribution from spiny stellates than previously shown for V2 overall, indicating that a larger amount of M information than previously thought flows into both the dorsal and ventral streams via the V2 thick and thin stripes, respectively. Moreover, we identify four types of V2-projecting L4B cells differing in size and complexity. Three such cell types project to both thin and thick stripes, but one type, the giant spiny-stellate neuron, resembling L4B neurons projecting to motion-sensitive area MT, was only found to project to thick stripes.SIGNIFICANCE STATEMENT Area V1 partitions visual information into functionally specialized parallel pathways which terminate into distinct stripes of area V2. We asked whether V1 inputs to different V2 stripes arise from morphologically different cell types. V1 layer (L)4B has two cell types: pyramids, which carry both magnocellular (M) and parvocellular (P) visual signals, and spiny stellates, which carry only M signals. We find that V2 thick stripes, which project to areas processing object motion, receive a larger fraction of L4B input from M-dominated stellates compared with thin stripes, which project to areas processing object attributes. We also identify four morphological types of V2-projecting L4B neurons, suggestive of four functionally specialized cell types.