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Updated: Jan 21, 2026

Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants
Published on: October 15, 2019
Promoting Primary Myoblast Differentiation Through Retinoid X Receptor Signaling
Jihong Chen1, Qiao Li2,3
1Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
Abstract:
The differentiation and fusion of primary myoblasts into myotubes is tightly regulated through muscle-specific transcription networks and can be enhanced by small molecular inducers, which allow us to identify novel genetic targets and molecular interactions. As the pressing issue is to develop pharmacotherapy to prevent and treat muscle-related diseases, we describe how to efficiently direct the differentiation of primary myoblasts by using a nuclear receptor agonist for the development of muscle therapeutics.
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