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Updated: Jan 21, 2026

Techniques to Induce and Quantify Cellular Senescence
06:51

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

34.9K

[Ginsenoside Rg_1 induces leukemia stem cell senescence via SIRT1/TSC_2 signal axis]

Yan-Long Tang1, Yue Zhou2, Cheng-Gui Zhang2

  • 1the First Affiliated Hospital of Dali University Dali 671000, China.

Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica
|July 31, 2019
PubMed

Insights

Ginsenoside Rg_1 induces senescence in leukemia stem cells by downregulating the SIRT1/TSC_2 signaling pathway. This natural compound inhibits proliferation and self-renewal, offering potential therapeutic strategies for leukemia.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Leukemia stem cells (LSCs) are crucial for leukemia development and resistance.
  • Targeting LSC senescence is a promising therapeutic strategy.
  • Ginsenoside Rg_1 is a natural compound with potential anti-cancer properties.

Purpose of the Study:

  • To investigate the effect of ginsenoside Rg_1 on inducing senescence in CD34+CD38- leukemia stem cells (LSCs).
  • To explore the role of the SIRT1/TSC_2 signaling axis in Rg_1-induced LSC senescence.

Main Methods:

  • Isolation of CD34+CD38- LSCs using magnetic cell sorting (MACS).
  • Induction of senescence using ginsenoside Rg_1.
  • Evaluation of senescence via SA-β-Gal staining, cell cycle analysis, CCK-8, and colony formation assays.
  • Assessment of SIRT1 and TSC_2 expression using quantitative PCR and Western blotting.

Main Results:

  • Ginsenoside Rg_1 effectively induced senescence in CD34+CD38- LSCs, evidenced by increased SA-β-Gal staining and morphological changes.
  • Rg_1 inhibited LSC proliferation and self-renewal, increasing G0/G1 phase arrest and reducing colony formation.
  • Expression of SIRT1 and TSC_2 mRNA and protein was significantly downregulated by Rg_1 treatment.

Conclusions:

  • Ginsenoside Rg_1 induces senescence in leukemia stem cells.
  • The SIRT1/TSC_2 signaling pathway plays a significant role in ginsenoside Rg_1-mediated LSC senescence.
  • These findings suggest Rg_1 as a potential therapeutic agent for leukemia targeting LSCs.
Keywords:
CD34~+CD38~-LSCsSIRT1TSC_2ginsenoside Rg_1senescence

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