Related Experiment Video
Updated: Jan 21, 2026

A Micro-agar Salt Bridge Electrode for Analyzing the Proton Turnover Rate of Recombinant Membrane Proteins
Published on: January 7, 2019
Stability of buried and networked salt-bridges (BNSB)in thermophilic proteins
Amal Kumar Bandyopadhyay1, Rifat Nawaz Ul Islam2, Debanjan Mitra1
1Department of Biotechnology, University of Burdwan, Burdwan, West Bengal,India.
Abstract:
Thermophilic proteins function at high temperature, unlike mesophilic proteins. Thermo-stability of these proteins is due to the unique buried and networked salt-bridge (BNSB). However, it is known that the desolvation cost of BNSB is too high compared to other favorable energy terms. Nonetheless, it is known that stability is provided generally by hydrophobic isosteres without the need for BNSB. We show in this analysis that the BNSB is the optimal evolutionary design of salt-bridge to offset desolvation cost efficiently. Hence, thermophilic proteins with a high level of BNSB provide thermo-stability.
More Related Videos
07:22How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
Published on: February 11, 2019
06:50Marble Burying and Nestlet Shredding as Tests of Repetitive, Compulsive-like Behaviors in Mice
Published on: December 24, 2013
Related Concept Videos
Protein Networks
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks
Determining the pH of Salt Solutions
Responses to Salt Stress
Design Example: Strain Gauge Bridge or Wheatstone Bridge
RNA Stability