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The Progress of CRISPR/Cas9-Mediated Gene Editing in Generating Mouse/Zebrafish Models of Human Skeletal Diseases

Nan Wu1,2,3, Bowen Liu1,2, Huakang Du1,2

  • 1Department of Orthopedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.

Computational and Structural Biotechnology Journal
|July 31, 2019
PubMed

Insights

Genetic factors significantly impact skeletal diseases affecting development and metabolism. The CRISPR/Cas9 system accelerates the creation of mouse and zebrafish models for studying these genetic causes.

Area of Science:

  • Genetics
  • Developmental Biology
  • Orthopedics

Background:

  • Skeletal diseases stem from genetic factors impacting bone development, metabolism, and cellular processes.
  • Traditional methods for creating genetic animal models are time-consuming and expensive.
  • Advancements in gene-editing technologies, particularly CRISPR/Cas9, offer faster and more efficient model generation.

Purpose of the Study:

  • To review the application of CRISPR/Cas9 in generating mouse and zebrafish models for human skeletal diseases.
  • To highlight the contribution of these models in understanding disease mechanisms.

Main Methods:

  • Review of literature on CRISPR/Cas9-generated mouse and zebrafish models.
  • Analysis of studies investigating genetic factors in skeletal development and metabolism.

Main Results:

  • CRISPR/Cas9 has significantly accelerated the creation of genetically modified mouse and zebrafish models.
  • These models are crucial for functional studies of genes and variants implicated in skeletal diseases.
  • The review covers models for various skeletal defects, including ossification, growth, and signaling pathway disturbances.

Conclusions:

  • CRISPR/Cas9 technology is revolutionizing the study of genetic skeletal diseases.
  • Mouse and zebrafish models generated via CRISPR/Cas9 are invaluable tools for deciphering disease underpinnings.
  • This approach facilitates a deeper understanding of skeletal development, metabolism, and related disorders.

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