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Related Experiment Video

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Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
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Microsatellite Marker Analysis for Laboratory Mice Profiling.

Reza Ahangari Cohan1, Davoud Nouri Inanlou2, Mohammad Hasan Samiee Aref3

  • 1Department of Nanobiotechnology, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran.

Advanced Biomedical Research
|July 31, 2019
PubMed
Summary

Genetic profiling using microsatellite markers offers a reliable method for confirming the genetic purity of inbred laboratory mice. This DNA-based approach surpasses traditional methods for quality control in animal research.

Keywords:
BALB/cJgenetic profileinbredlaboratory micemicrosatellite

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Area of Science:

  • Genetics
  • Animal Science
  • Molecular Biology

Background:

  • Ensuring genetic purity is crucial in producing inbred animals.
  • Traditional methods like skin grafting and coat color tests have limitations.
  • DNA profiling offers an advanced solution for verifying animal genetic purity.

Purpose of the Study:

  • To develop and validate microsatellite markers for assessing the genetic purity of inbred laboratory mice.
  • To establish a DNA-based method for quality control in laboratory animal production.

Main Methods:

  • Twenty microsatellite markers were designed across 20 chromosomes.
  • Primers were distinguished by color and size for clear identification.
  • These markers were used in polymerase chain reaction (PCR) to analyze inbred BALB/cJ strains.

Main Results:

  • The 20 designed microsatellite markers effectively identified the inbred BALB/cJ mouse strain.
  • The study confirmed the suitability of these markers for genetic purity testing.
  • Distinct genetic profiles were generated for parentage analysis.

Conclusions:

  • Microsatellite marker-based genetic profiling is a powerful tool for quality control in laboratory animal research.
  • This method enables accurate detection of genetic variations in mouse species.
  • It enhances validation processes in laboratory animal production.