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Glucose and pH Dual-Responsive Nanogels for Efficient Protein Delivery
Xinghui Si1,2, Wantong Song1,3, Shengcai Yang1,4
1Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
Macromolecular Bioscience
|July 31, 2019
Summary
New biodegradable nanogels offer a solution for unstable protein delivery. These smart nanogels respond to pH and glucose levels, ensuring protein stability and controlled release for therapeutic applications.
Area of Science:
- Biomaterials Science
- Drug Delivery Systems
- Nanotechnology
Background:
- Direct protein delivery faces challenges due to instability, immunogenicity, and short half-life.
- Existing methods struggle to maintain protein integrity and achieve controlled release in vivo.
Purpose of the Study:
- To design pH and glucose dual-responsive biodegradable nanogels for enhanced protein delivery.
- To overcome the limitations of direct protein administration by improving stability and controlling release.
Main Methods:
- Synthesized dual-responsive nanogels using dextran and poly(L-glutamic acid)-g-methoxy poly-(ethylene glycol)/phenyl boronic acid (PLG-g-mPEG/PBA).
- Evaluated drug-loading capacity for α-amylase and hyaluronidase.
- Assessed in vitro protein release profiles under varying pH and glucose concentrations.
- Analyzed the secondary structure of released proteins using circular dichroism spectroscopy.
Main Results:
- Achieved high drug-loading efficacy: 55.6% for α-amylase and 29.1% for hyaluronidase.
- Demonstrated pH and glucose-dependent protein release: minimal release at pH 7.4/low glucose, accelerated release at pH 5.5/high glucose.
- Confirmed that the secondary structure of released proteins is preserved, indicating maintained protein integrity.
Conclusions:
- Developed a novel nanogel system for effective protein delivery.
- The dual-responsive nanogels provide a promising strategy to enhance protein stability and enable controlled release based on physiological cues.
- This approach offers a simple and effective solution for overcoming protein delivery challenges.
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