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Published on: March 24, 2017
Linear Peptides Containing d-Leucine with Neuroprotective Activities from the Leech Whitmania pigra Whitman
Zi-Yue Zhang1,2, Nan Ma1,2, Li-Jun Tao1,2
1Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy , Jinan University , Guangzhou 510632 , People's Republic of China.
Abstract:
Three new linear peptides containing d-leucine, named whitmantides A-C (1-3), were isolated from the dried whole bodies of Whitmania pigra Whitman. Their structures with absolute configurations were elucidated by Edman degradation, mass spectrometry, Marfey's analysis, and solid-phase synthesis. It is the first time that peptides containing d-amino acid in leeches were discovered. Compounds 1-3 displayed neuroprotective activities against oxygen-glucose deprivation/reperfusion injury on Neuro-2a cells. In addition, ex vivo serum stability tests showed that 1-3 were resistant to protease degradation.
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