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A Laboratory-Built Fully Automated Ultrasonication Robot for Filamentous Fungi Homogenization.

Ya Xiong1, Volha Shapaval1, Achim Kohler1

  • 1Faculty of Science and Technology, Norwegian University of Life Sciences, Ås, Norway.

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Summary

A new hands-free robot automates filamentous fungi homogenization using ultrasonication. Machine vision and feedback control ensure efficient and consistent sample preparation, reducing manual intervention.

Keywords:
fungi homogenization measurementfungi homogenization modelinglaboratory automationroboticsultrasonication

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Area of Science:

  • Biotechnology
  • Robotics
  • Microbiology

Background:

  • Filamentous fungi homogenization is crucial for downstream applications.
  • Manual homogenization methods are labor-intensive and can introduce variability.

Purpose of the Study:

  • To design and develop a novel hands-free ultrasonication robot for automated filamentous fungi homogenization.
  • To implement machine vision for real-time monitoring and control of the homogenization process.

Main Methods:

  • Construction of a robot using a modified 3D printer with integrated camera, wash station, and sonicator.
  • Application of machine vision for sample screening and homogeneity assessment via color variance.
  • Development of a feedback control system using standard deviation of homogeneity levels to determine termination time.

Main Results:

  • The ultrasonication homogenization process follows an exponential decay model, with rapid homogenization initially.
  • Higher sonicator power and temperature accelerate homogenization; cultivation time has no significant effect.
  • A feedback control system using standard deviation effectively determined termination time for automated operation.

Conclusions:

  • The developed hands-free robot efficiently automates filamentous fungi homogenization.
  • Machine vision and feedback control enable precise and consistent sample preparation.
  • This automated system significantly reduces manual labor and improves experimental reproducibility.