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Administration of an aldose reductase inhibitor induces a decrease of collagen fluorescence in diabetic rats
G Suárez1, R Rajaram, K C Bhuyan
1Department of Orthopaedics, Mount Sinai School of Medicine, New York 10029.
The Journal of Clinical Investigation
|August 1, 1988
Summary
High fructose levels in diabetes contribute to protein damage. Aldose reductase inhibitor treatment reduced this damage in diabetic rats, suggesting a role in diabetic complications.
Area of Science:
- Biochemistry
- Endocrinology
- Diabetic Complications
Background:
- Diabetes elevates sorbitol pathway flux, increasing tissue fructose levels.
- Nonenzymatic protein fructosylation generates fluorescence, a marker of advanced glycation end products.
- This process occurs at a significantly higher rate with fructose compared to glucose.
Purpose of the Study:
- To investigate the role of nonenzymatic fructosylation in diabetic complications.
- To determine if aldose reductase inhibition affects in vivo protein fructosylation.
Main Methods:
- Administered sorbinil, an aldose reductase inhibitor, to experimental diabetic rats.
- Measured fluorescence in skin collagen, indicative of advanced Maillard products.
Main Results:
- Sorbinil administration lowered tissue fructose concentration.
- A significant decrease in skin collagen fluorescence was observed in treated diabetic rats.
- This reduction suggests decreased in vivo nonenzymatic fructosylation of collagen.
Conclusions:
- Nonenzymatic fructosylation of collagen occurs in vivo in diabetic conditions.
- Aldose reductase inhibition may mitigate diabetic complications by reducing protein fructosylation.
- Further research is warranted to explore the pathogenetic role of this posttranslational modification.
