Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Plant Cells and Tissues02:01

Plant Cells and Tissues

65.2K
Plant tissues are collections of similar cells performing related functions. Different plant tissues will have their own specialized roles and can be combined with other tissues to form organs such as flowers, fruit, stem, and leaves. Two major types of plant tissue include meristematic and permanent tissue.
65.2K
What is Metabolism?00:52

What is Metabolism?

131.2K
Overview
131.2K
Subcellular Fractionation01:32

Subcellular Fractionation

8.6K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
8.6K
Plant Hormones01:56

Plant Hormones

27.4K
Plant hormones—or phytohormones—are chemical molecules that modulate one or more physiological processes of a plant. In animals, hormones are often produced in specific glands and circulated via the circulatory system. However, plants lack hormone-producing glands.
27.4K
Tonicity in Plants00:53

Tonicity in Plants

59.7K
Tonicity describes the capacity of a cell to lose or gain water. It depends on the quantity of solute that does not penetrate the membrane. Tonicity delimits the magnitude and direction of osmosis and results in three possible scenarios that alter the volume of a cell: hypertonicity, hypotonicity, and isotonicity. Due to differences in structure and physiology, tonicity of plant cells is different from that of animal cells in some scenarios.
59.7K
Plant Cell Wall02:43

Plant Cell Wall

60.1K
The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.
60.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A metabolic model of the leaf-associated bacterium <i>Paraburkholderia dioscoreae</i> links the ethylene precursor ACC to core carbon pathways.

mSystems·2026
Same author

Ecological role of emergent properties in the chemodiversity landscape.

Nature ecology & evolution·2026
Same author

PHR1 mediates rapid high light responses and acclimation to high photosynthetic activity.

The Plant journal : for cell and molecular biology·2026
Same author

Clusia genomes shed light on the evolution and diversity of crassulacean acid metabolism physiotypes.

Nature communications·2026
Same author

Metabolic Snorkeling - The Metabolic Interaction Between Plant Organs and Tissues with different Oxygen Availabilities.

Journal of experimental botany·2026
Same author

Microbiome assembly statistics toward ecosystem-scale insights, forecasting, and management.

The ISME journal·2026

Related Experiment Video

Updated: Jan 21, 2026

Elucidating the Metabolism of 2,4-Dibromophenol in Plants
06:54

Elucidating the Metabolism of 2,4-Dibromophenol in Plants

Published on: February 10, 2023

1.5K

Resolving subcellular plant metabolism.

Lisa Fürtauer1,2, Lisa Küstner3, Wolfram Weckwerth2,4

  • 1Department Biology I, Plant Evolutionary Cell Biology, Ludwig-Maximilians-Universität München, Planegg-Martinsried, Germany.

The Plant Journal : for Cell and Molecular Biology
|July 31, 2019
PubMed
Summary

This study reveals plant cell metabolism dynamics using combined proteomics and metabolomics. HXK1 activity impacts glucose distribution and photorespiratory capacity in different cell compartments.

Keywords:
Arabidopsis thalianahexokinase 1metabolomicsnonaqueous fractionationphotorespirationproteomicssubcellular metabolism

More Related Videos

Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins
08:39

Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins

Published on: December 24, 2017

7.5K
Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling
10:16

Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling

Published on: January 16, 2014

23.5K

Related Experiment Videos

Last Updated: Jan 21, 2026

Elucidating the Metabolism of 2,4-Dibromophenol in Plants
06:54

Elucidating the Metabolism of 2,4-Dibromophenol in Plants

Published on: February 10, 2023

1.5K
Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins
08:39

Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins

Published on: December 24, 2017

7.5K
Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling
10:16

Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling

Published on: January 16, 2014

23.5K

Area of Science:

  • Plant cell biology
  • Molecular plant science
  • Biochemistry

Background:

  • Plant cells exhibit complex compartmentalization of proteomes and metabolomes.
  • Limited studies combine subcellular proteomics and metabolomics, hindering interpretation of 'omics data.
  • Understanding subcellular metabolic dynamics is crucial for plant physiology.

Purpose of the Study:

  • To develop and apply a method for analyzing subcellular diurnal dynamics of plant metabolism.
  • To investigate the impact of HXK1 activity on subcellular metabolome and proteome dynamics.
  • To establish a causal link between metabolites, proteins, and metabolic regulation at the subcellular level.

Main Methods:

  • Combined nonaqueous fractionation, shotgun proteomics, enzyme activities, and metabolomics.
  • Identified and enzymatically validated subcellular marker protein sets for a four-compartment model (chloroplasts, cytosol, vacuole, mitochondria).
  • Compared wild type plants with HXK1-deficient gin2-1 mutants.

Main Results:

  • Developed validated marker sets for monitoring subcellular dynamics.
  • HXK1 activity significantly impacts metabolome dynamics across multiple compartments.
  • Observed altered glucose distribution, pyruvate, succinate, fumarate levels, and mitochondrial proteome dynamics in gin2-1 mutants.
  • Reduced glycine and serine levels, and decreased photorespiratory proteins in gin2-1 mutants suggest impaired photorespiratory capacity.

Conclusions:

  • The study presents a robust methodological framework for subcellular plant metabolism analysis.
  • HXK1 plays a critical role in regulating glucose homeostasis and metabolic flux in plant cells.
  • Resolving plant metabolism to the subcellular level is essential for understanding metabolic regulation and physiological responses.