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Updated: Jan 21, 2026

Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion
Published on: February 24, 2021
Platelet-rich plasma affects the proliferation of canine bone marrow-derived mesenchymal stromal cells in vitro
Sony Pandey1, Dawn U Hickey1, Marti Drum1
1Department of Small Animal Clinical Siences, College of Veterinary Medicine, The University of Tennessee, Knoxville, 37996, TN, USA.
Background:
Reported efficacy of platelet-rich plasma (PRP) in regenerative medicine is contradictory. We validated the effects of PRP on proliferation of canine bone marrow-derived multipotent mesenchymal stromal cells (K9BMMSCs) in vitro. PRP was extracted from blood of six dogs with osteoarthritis. K9BMMSCs were established from bone marrow and characterized for CD90 and CD19 expression by immunocytochemistry. Effects of PRP concentrations on viability of matching autologous K9BMMSCs were validated using MTS assay.
Results:
Positive CD90 and negative CD19 expression confirmed MSC origin. PRP at 40% volume/volume concentration increased, while PRP at 80 and 100% v/v concentrations suppressed viability of tested K9BMMSCs.
Conclusion:
PRP concentration plays an important role in K9BMMSCs viability, which could affect tissue repairs in vivo.
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