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[Changes in two-dimensional gel electrophoretic patterns of cellular protein spots in tumors after administration of
1Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
Abstract:
The changes of cellular protein spots in human stomach carcinomas (Exp 4, H-111) and colon carcinomas (SW403, SW480) implanted into nude mice were examined after administration of MMC. The results were as follows. 1) Exp4 histologically showed pleomorphism after treatment by MMC. In other tumors no changes in histological features were shown. 2) The most remarkable decreases of p protein spots in Exp4, and moderate decreases of protein spots in SW403 and SW480 were admitted, contrasting to no changes in the pattern of protein spots in H-111. 3) The decreases of protein spots in the tumors were related to the sensitivities to MMC. 4) Regrowth tumors in Exp4 which were survived after administration of MMC revealed similar protein spots patterns and histological features to those in control tumors. 5) The changes in protein spots in the liver and kidney derived from normal mice were also examined. In these, protein spots patterns showed no changes after administration of MMC. These results suggest that the cellular protein sports were decreased in accordance with effect of MMC in the tumors.