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Related Experiment Video

Updated: Jan 21, 2026

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ZMYM2 inhibits NANOG-mediated reprogramming.

Moyra Lawrence1,2, Thorold W Theunissen1,2,3, Patrick Lombard1

  • 1Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, Cambridgeshire, CB2 1QR, UK.

Wellcome Open Research
|August 1, 2019
PubMed
Summary

ZMYM2 negatively regulates NANOG-mediated reprogramming and impairs embryonic stem cell self-renewal. This transcription factor acts as a key interactor, curtailing NANOG

Keywords:
NanogZinc finger proteindifferentiationpluripotencyreprogramming

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Area of Science:

  • Stem cell biology
  • Molecular and cellular biology
  • Epigenetics and gene regulation

Background:

  • NANOG is a key transcription factor in the pluripotency network, crucial for reprogramming cells to a naïve state.
  • NANOG interacts with various partners, influencing its reprogramming capabilities.
  • Understanding NANOG interactors is vital for optimizing reprogramming efficiency.

Purpose of the Study:

  • To investigate the impact of NANOG interactors on the reprogramming process.
  • To identify specific NANOG-binding proteins that modulate reprogramming efficiency.
  • To analyze the role of ZMYM2 in NANOG-mediated reprogramming and stem cell self-renewal.

Main Methods:

  • Reprogramming of epiblast stem cells and somatic cells to naïve pluripotency using 2i/LIF.
  • CRISPR/Cas9 gene editing for ZMYM2 knockout and PiggyBac system for overexpression.
  • Quantification of reprogramming efficiency and embryonic stem cell self-renewal in differentiation assays.

Main Results:

  • ZMYM2/ZFP198 was identified as a physical interactor of NANOG.
  • ZMYM2 acts as a negative regulator of NANOG-mediated reprogramming in both cell types.
  • ZMYM2 overexpression impairs embryonic stem cell self-renewal and promotes differentiation.

Conclusions:

  • ZMYM2 significantly curtails NANOG's function in reprogramming somatic and epiblast stem cells.
  • ZMYM2 impedes embryonic stem cell self-renewal, promoting cellular differentiation.
  • ZMYM2 is a critical regulator controlling pluripotency maintenance and reprogramming outcomes.