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FSH1 overexpression triggers apoptosis in Saccharomyces cerevisiae

Ramachandran Gowsalya1, Chidambaram Ravi1, Mathivanan Arul1

  • 1Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620024, India.

Antonie Van Leeuwenhoek
|August 1, 2019
PubMed

Insights

FSH1 gene overexpression in yeast induces cell death, mimicking mammalian apoptosis. Deleting FSH1 increases cell growth and reduces apoptosis markers, suggesting FSH1 regulates cell death pathways.

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Cell Death Research

Background:

  • FSH1 is a serine hydrolase in yeast, homologous to human OVAC2.
  • Preliminary data suggests Fsh1p influences cell growth and apoptosis gene expression.

Purpose of the Study:

  • To investigate the role of FSH1 in yeast cell death.
  • To determine if FSH1 overexpression induces apoptosis-like phenotypes in yeast.

Main Methods:

  • Gene knockout and overexpression in yeast (Saccharomyces cerevisiae).
  • Analysis of cell growth rates.
  • Assessment of apoptosis markers: DNA fragmentation, phosphatidylserine externalization, ROS accumulation, Cytochrome c release, mitochondrial membrane potential.

Main Results:

  • FSH1 deletion increased cell growth and decreased apoptosis-responsive genes (AIF1, NUC1).
  • FSH1 overexpression inhibited growth and induced mammalian-like apoptosis markers.
  • Overexpression of FSH1 upregulated AIF1 levels in wild-type cells.

Conclusions:

  • FSH1 plays a critical role in regulating cell death in yeast.
  • FSH1 overexpression can induce apoptosis through conserved molecular pathways.
  • FSH1 is a potential target for modulating cell death in yeast models.
Keywords:
ApoptosisCell deathFSHOVCA2

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