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Profiling of Cytokine and Chemokine Responses Using Multiplex Bead Array Technology

Greg Harris1, Wangxue Chen2

  • 1Human Health and Therapeutics Research Center, National Research Council of Canada, Ottawa, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|August 1, 2019
PubMed

Multiplex bead array technology expands upon the principles of the enzyme-linked immunosorbent assay by allowing the simultaneous quantification of a large number of cytokines and chemokines within a single sample. This allows researchers more freedom and opportunities to investigate complex immune responses both in vivo and in vitro. Here we describe and update the detailed assay protocol and technical tips for simultaneous quantification of multiple cytokines and chemokines in mouse biological fluids such as sera, bronchoalveolar lavage fluid, tissue homogenate supernatant, and tissue culture supernatant, using a multiplex bead array assay.

Keywords:
Bead arrayChemokineCytokineImmunoassayLuminexMultiplex assay

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