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Antigen Identification for Cell-Binding Antibodies Using Ligand-Directed Crosslinking and Biotin Transfer

Tammy-Lynn Tremblay1, Jennifer J Hill2

  • 1Human Health Therapeutics, National Research Council Canada, Ottawa, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|August 1, 2019
PubMed
Summary

This study introduces a new method using ligand-directed crosslinking and proteomic analysis to identify unknown cellular receptors for orphan ligands. The protocol helps pinpoint specific cell surface targets for antibodies identified through antibody library panning.

Keywords:
AntibodiesCrosslinkingMass spectrometryProteomicsReceptor identification

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